ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of MICA/B in human esophageal cancer, and to analyze its correlation with clinicopathological features.</p><p><b>METHODS</b>The expression of MICA/B in 40 cases of esophagus carcinoma and corresponding normal esophageal mucosa tissues were examined by immunohistochemistry.</p><p><b>RESULTS</b>The positive rate of expression of MICA/B protein in the esophageal carcinoma was 75.0% (30/40), and that in the corresponding normal esophageal mucosa was 0 (0/40). Up-regulation of MICA/B expression was found in the esophageal carcinomas. The expression of MICA/B was related with histological grade of the esophageal carcinoma (P = 0.012).</p><p><b>CONCLUSION</b>MICA/B protein plays an important role in the esophageal carcinogenesis, and my become a useful molecular marker for the diagnosis of esophageal carcinoma.</p>
Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Biomarkers, Tumor , Metabolism , Esophageal Neoplasms , Diagnosis , Metabolism , Pathology , Histocompatibility Antigens Class I , Metabolism , Immunohistochemistry , Neoplasm Grading , Up-RegulationABSTRACT
<p><b>OBJECTIVE</b>To evaluate the growth-inhibiting and pro-apoptotic effect of kaempferol in human esophageal squamous carcinoma Eca-109 cells and explore the mechanism.</p><p><b>METHODS</b>The effect of kaempferol on Eca-109 cell proliferation in vitro was measured by MTT assay. TUNEL staining was used to detect the cell apoptosis following kaempferol treatment. The changes in Bax and Bcl-2 mRNA expressions in response to kaempferol treatment were determined by RT-PCR, and the caspase-3 and caspase-9 activities were evaluated using colorimetric assay.</p><p><b>RESULTS</b>Kaempferol significantly inhibited Eca-109 cell proliferation (P<0.05) in a concentration-dependent manner and induced obvious cell apoptosis. RT-PCR showed that after kaempferol treatment caused up-regulated Bax and down-regulated Bcl-2 mRNA expression. The colorimetric assay revealed significantly increased caspase-3 and caspase-9 activities in Eca-109 cells following kaempferol treatment (P<0.01).</p><p><b>CONCLUSION</b>Kaempferol can induce apoptosis of Eca-109 cells via a mitochondria-dependent pathway.</p>